Various kinds of medicinal herbs and prescribed herb medicines which have usually been used for treatment of reducing fever, purging intense heat, and detoxication were screened for antibacterial activity against G.. vaginalis which causing bacterial vaginosis. Minimal inhibitory concentrations of water- and ethanol-soluble extracts of Omiza (Schizandra chinensis) and Omae (Prunus mume) were determined. Using thin-layer chromatography, water- and ethanol-soluble extracts of Omiza (S. chinensis) and Omae (P, mume) separated. The results obtained were as follows:
1. Among the 36 medicinal herbs tested, the water-soluble extracts of Omiza (S. chinensis), Omae (P. mume), Hwangkeum (S. baicalensis), Seungma (C heracleifolia), and Dansam (S. miltiorrhiza) showed antibacterial activities against G. vaginalis; the extract of Omiza A chinensis) and Omae (P. mume) inhibited cell growth of G vaginalis much more than those of the others did. The water-soluble extract of Omiza (S. chinensis) and those of both Omiza (S chinensis) and Omae (P. mume) showed antibacterial activity against E coli and against E coli. and B. subtilis, respectively.
2. The water- and ethanol-soluble extracts of Omiza (S chinensis) and Omae (P. mume) showed excellent antibacterial activities.
3. With the water-soluble extracts of both Orniza (S. chinensis) and Omae (P. mume), minimal inhibitory concentrations (MICs) against G. vaginalis were 1.13 and 5.0 ㎎/ml, respectively. With the ethanol-soluble extracts of both, MICs were 2.5 ㎎/ml.
4. Among the prescribed herb medicines tested, Chusineum, Ilsihoeum, Daebunchongeum, Samsihoeum, and Osihoeum showed antibacterial activities against G. vaginalis. Chusinseum and Ilsihoeum were excellent, with respect to antibacterial activity.
5. The water- and sthanol-soluble extracts of both Omiza (S chinensis) and Omae (P. mume) were separated using Thin-layer chromatography and detected with UV-detector. Further study should be carried out to identify which compound affects cell growth inhibition of G vaginalis.
As a result, the antibacterial compounds against a pathogenic bacteria, G vaginalis, are contained in the water- and ethanol-soluble extracts of Omiza (S. chinensis) and Omae (P. mume) and the prescribed herb medicines, Chusineum and Ilsihoeum, and may be used for treatment of vaginosis-causing G vaginalis.
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